Induction of squamous differentiation of normal human bronchial epithelial cells by small amounts of serum
JF Lechner, A Haugen, IA McClendon… - Differentiation, 1984 - Elsevier
Recently we reported a low calcium (110 μM) serum-free medium (LHC-1) for clonal growth
of normal human bronchial epithelial (NHBE) cells. NHBE cells within colonies are small
(mean surface area= 1,250 μ 2) rarely migratory, have few tonofilaments, and multiply with
an average population doubling time of 28 h. We have also noted that adding small amounts
of blood-derived serum to LHC-1 medium (as little as 2%) significantly decreased the clonal
growth rate. We have now found that the growth inhibiting effect of serum is due to the …
of normal human bronchial epithelial (NHBE) cells. NHBE cells within colonies are small
(mean surface area= 1,250 μ 2) rarely migratory, have few tonofilaments, and multiply with
an average population doubling time of 28 h. We have also noted that adding small amounts
of blood-derived serum to LHC-1 medium (as little as 2%) significantly decreased the clonal
growth rate. We have now found that the growth inhibiting effect of serum is due to the …
